Construct: shRNA TRCN0000314594

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003672.3-2451s21c1
DNA Barcode:
GACTACTATAAATGCACTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDC14A (8556)
Gene Description:
cell division cycle 14A
Transcript:
RefSeq NM_003672.3 (NON-CURRENT)
Match location:
Position 2451 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000314594)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTACTATAAATGCACTGAA
Hairpin Sequence:
5'-CCGG-GACTACTATAAATGCACTGAA-CTCGAG-TTCAGTGCATTTATAGTAGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTACTATAAATGCACTGAACTCGAGTTCAGTGCATTTATAGTAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTACTATAAATGCACTGAACTCGAGTTCAGTGCATTTATAGTAGTC-3'

Other clones with same target sequence:

  1. TRCN0000002977