Construct: shRNA TRCN0000314654

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004654.3-8641s21c1
DNA Barcode:
GACTATGCACTAAGGTCTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
USP9Y (8287)
Gene Description:
ubiquitin specific peptidase 9 Y-linked
Transcript:
RefSeq NM_004654.3 (NON-CURRENT)
Match location:
Position 8641 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000314654)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTATGCACTAAGGTCTTAT
Hairpin Sequence:
5'-CCGG-GACTATGCACTAAGGTCTTAT-CTCGAG-ATAAGACCTTAGTGCATAGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTATGCACTAAGGTCTTATCTCGAGATAAGACCTTAGTGCATAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTATGCACTAAGGTCTTATCTCGAGATAAGACCTTAGTGCATAGTC-3'

Other clones with same target sequence:

(none)