Construct: shRNA TRCN0000314656

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000044.2-2969s21c1
DNA Barcode:
GAAATGTTATGAAGCAGGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AR (367)
Gene Description:
androgen receptor
Transcript:
RefSeq NM_000044.2 (NON-CURRENT)
Match location:
Position 2969 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000314656)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAATGTTATGAAGCAGGGAT
Hairpin Sequence:
5'-CCGG-GAAATGTTATGAAGCAGGGAT-CTCGAG-ATCCCTGCTTCATAACATTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAATGTTATGAAGCAGGGATCTCGAGATCCCTGCTTCATAACATTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAATGTTATGAAGCAGGGATCTCGAGATCCCTGCTTCATAACATTTC-3'

Other clones with same target sequence:

  1. TRCN0000003716