Construct: shRNA TRCN0000314824

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005975.2-684s21c1
DNA Barcode:
AGGTGGCCATTAAGGTGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTK6 (5753)
Gene Description:
protein tyrosine kinase 6
Transcript:
RefSeq NM_005975.2 (NON-CURRENT)
Match location:
Position 684 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000314824)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGTGGCCATTAAGGTGATTT
Hairpin Sequence:
5'-CCGG-AGGTGGCCATTAAGGTGATTT-CTCGAG-AAATCACCTTAATGGCCACCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGTGGCCATTAAGGTGATTTCTCGAGAAATCACCTTAATGGCCACCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGTGGCCATTAAGGTGATTTCTCGAGAAATCACCTTAATGGCCACCT-3'

Other clones with same target sequence:

(none)