Construct: shRNA TRCN0000314887

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014496.2-1555s21c1
DNA Barcode:
TTTACCTTGTTACGGATTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPS6KA6 (27330)
Gene Description:
ribosomal protein S6 kinase A6
Transcript:
RefSeq NM_014496.2 (NON-CURRENT)
Match location:
Position 1555 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000314887)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTTACCTTGTTACGGATTTAA
Hairpin Sequence:
5'-CCGG-TTTACCTTGTTACGGATTTAA-CTCGAG-TTAAATCCGTAACAAGGTAAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTTACCTTGTTACGGATTTAACTCGAGTTAAATCCGTAACAAGGTAAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTTACCTTGTTACGGATTTAACTCGAGTTAAATCCGTAACAAGGTAAA-3'

Other clones with same target sequence:

(none)