Construct: shRNA TRCN0000314891

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016440.3-1087s21c1
DNA Barcode:
CGGTGTTCACCAGGACAAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VRK3 (51231)
Gene Description:
VRK serine/threonine kinase 3
Transcript:
RefSeq NM_016440.3 (NON-CURRENT)
Match location:
Position 1087 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000314891)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTGTTCACCAGGACAAATA
Hairpin Sequence:
5'-CCGG-CGGTGTTCACCAGGACAAATA-CTCGAG-TATTTGTCCTGGTGAACACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTGTTCACCAGGACAAATACTCGAGTATTTGTCCTGGTGAACACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTGTTCACCAGGACAAATACTCGAGTATTTGTCCTGGTGAACACCG-3'

Other clones with same target sequence:

(none)