Construct: shRNA TRCN0000314925

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016508.3-735s21c1
DNA Barcode:
GATTTGGATTTACTCCATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDKL3 (51265)
Gene Description:
cyclin dependent kinase like 3
Transcript:
RefSeq NM_016508.3 (CURRENT)
Match location:
Position 735 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000314925)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTTGGATTTACTCCATAAA
Hairpin Sequence:
5'-CCGG-GATTTGGATTTACTCCATAAA-CTCGAG-TTTATGGAGTAAATCCAAATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTTGGATTTACTCCATAAACTCGAGTTTATGGAGTAAATCCAAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTTGGATTTACTCCATAAACTCGAGTTTATGGAGTAAATCCAAATC-3'

Other clones with same target sequence:

(none)