Construct: shRNA TRCN0000314998

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030792.6-671s21c1
DNA Barcode:
TCTGGTGGGAAGTCCACAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GDPD5 (81544)
Gene Description:
glycerophosphodiester phosphodiesterase domain containing 5
Transcript:
RefSeq NM_030792.6 (NON-CURRENT)
Match location:
Position 671 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000314998)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTGGTGGGAAGTCCACAATG
Hairpin Sequence:
5'-CCGG-TCTGGTGGGAAGTCCACAATG-CTCGAG-CATTGTGGACTTCCCACCAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTGGTGGGAAGTCCACAATGCTCGAGCATTGTGGACTTCCCACCAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTGGTGGGAAGTCCACAATGCTCGAGCATTGTGGACTTCCCACCAGA-3'

Other clones with same target sequence:

(none)