Construct: shRNA TRCN0000315001

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003161.2-1493s21c1
DNA Barcode:
AGCACAGCAAATCCTCAGACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPS6KB1 (6198)
Gene Description:
ribosomal protein S6 kinase B1
Transcript:
RefSeq NM_003161.2 (NON-CURRENT)
Match location:
Position 1493 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000315001)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCACAGCAAATCCTCAGACA
Hairpin Sequence:
5'-CCGG-AGCACAGCAAATCCTCAGACA-CTCGAG-TGTCTGAGGATTTGCTGTGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCACAGCAAATCCTCAGACACTCGAGTGTCTGAGGATTTGCTGTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCACAGCAAATCCTCAGACACTCGAGTGTCTGAGGATTTGCTGTGCT-3'

Other clones with same target sequence:

  1. TRCN0000003159