Construct: shRNA TRCN0000315030

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001654.2-772s21c1
DNA Barcode:
GTCCACTCCCAACGTCCATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARAF (369)
Gene Description:
A-Raf proto-oncogene, serine/threonine kinase
Transcript:
RefSeq NM_001654.2 (NON-CURRENT)
Match location:
Position 772 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000315030)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCCACTCCCAACGTCCATAT
Hairpin Sequence:
5'-CCGG-GTCCACTCCCAACGTCCATAT-CTCGAG-ATATGGACGTTGGGAGTGGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCCACTCCCAACGTCCATATCTCGAGATATGGACGTTGGGAGTGGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCCACTCCCAACGTCCATATCTCGAGATATGGACGTTGGGAGTGGAC-3'

Other clones with same target sequence:

(none)