Construct: shRNA TRCN0000315043

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003743.4-4687s21c1
DNA Barcode:
CTTCAGGTCGTAGCATTTGGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NCOA1 (8648)
Gene Description:
nuclear receptor coactivator 1
Transcript:
RefSeq NM_003743.4 (NON-CURRENT)
Match location:
Position 4687 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000315043)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCAGGTCGTAGCATTTGGA
Hairpin Sequence:
5'-CCGG-CTTCAGGTCGTAGCATTTGGA-CTCGAG-TCCAAATGCTACGACCTGAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCAGGTCGTAGCATTTGGACTCGAGTCCAAATGCTACGACCTGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCAGGTCGTAGCATTTGGACTCGAGTCCAAATGCTACGACCTGAAG-3'

Other clones with same target sequence:

  1. TRCN0000004082