Construct: shRNA TRCN0000315055

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139283.1-550s21c1
DNA Barcode:
GGACGGTTCGTACCTAGTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPTC7 (160760)
Gene Description:
PTC7 protein phosphatase homolog
Transcript:
RefSeq NM_139283.1 (NON-CURRENT)
Match location:
Position 550 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000315055)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGACGGTTCGTACCTAGTAAT
Hairpin Sequence:
5'-CCGG-GGACGGTTCGTACCTAGTAAT-CTCGAG-ATTACTAGGTACGAACCGTCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGACGGTTCGTACCTAGTAATCTCGAGATTACTAGGTACGAACCGTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGACGGTTCGTACCTAGTAATCTCGAGATTACTAGGTACGAACCGTCC-3'

Other clones with same target sequence:

(none)