Construct: shRNA TRCN0000315073
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_005402.2-518s21c1
- DNA Barcode:
- AGGACTACGCTGCAATTAGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RALA (5898)
- Gene Description:
- RAS like proto-oncogene A
- Transcript:
- RefSeq NM_005402.2 (NON-CURRENT)
- Match location:
- Position 518 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000315073)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- AGGACTACGCTGCAATTAGAG
- Hairpin Sequence:
-
5'-CCGG-AGGACTACGCTGCAATTAGAG-CTCGAG-CTCTAATTGCAGCGTAGTCCT-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGAGGACTACGCTGCAATTAGAGCTCGAGCTCTAATTGCAGCGTAGTCCTTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAAGGACTACGCTGCAATTAGAGCTCGAGCTCTAATTGCAGCGTAGTCCT-3'
Other clones with same target sequence:
(none)
