Construct: shRNA TRCN0000315138

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002799.2-424s21c1
DNA Barcode:
ACATTGGTGCAGCCCTAGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PSMB7 (5695)
Gene Description:
proteasome 20S subunit beta 7
Transcript:
RefSeq NM_002799.2 (NON-CURRENT)
Match location:
Position 424 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000315138)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACATTGGTGCAGCCCTAGTTT
Hairpin Sequence:
5'-CCGG-ACATTGGTGCAGCCCTAGTTT-CTCGAG-AAACTAGGGCTGCACCAATGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACATTGGTGCAGCCCTAGTTTCTCGAGAAACTAGGGCTGCACCAATGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACATTGGTGCAGCCCTAGTTTCTCGAGAAACTAGGGCTGCACCAATGT-3'

Other clones with same target sequence:

  1. TRCN0000003927