Construct: shRNA TRCN0000315162
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_006758.2-837s21c1
- DNA Barcode:
- GAAAGTGTTGTAGTTGATTGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- U2AF1 (7307)
- Gene Description:
- U2 small nuclear RNA auxiliary factor 1
- Transcript:
- RefSeq NM_006758.2 (CURRENT)
- Match location:
- Position 837 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000315162)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GAAAGTGTTGTAGTTGATTGA
- Hairpin Sequence:
-
5'-CCGG-GAAAGTGTTGTAGTTGATTGA-CTCGAG-TCAATCAACTACAACACTTTC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGAAAGTGTTGTAGTTGATTGACTCGAGTCAATCAACTACAACACTTTCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGAAAGTGTTGTAGTTGATTGACTCGAGTCAATCAACTACAACACTTTC-3'
Other clones with same target sequence:
-
TRCN0000001155
