Construct: shRNA TRCN0000315172

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024652.3-6734s21c1
DNA Barcode:
CCCAGGTCTCAGATGGAATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LRRK1 (79705)
Gene Description:
leucine rich repeat kinase 1
Transcript:
RefSeq NM_024652.3 (NON-CURRENT)
Match location:
Position 6734 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000315172)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGGTCTCAGATGGAATTA
Hairpin Sequence:
5'-CCGG-CCCAGGTCTCAGATGGAATTA-CTCGAG-TAATTCCATCTGAGACCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGGTCTCAGATGGAATTACTCGAGTAATTCCATCTGAGACCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGGTCTCAGATGGAATTACTCGAGTAATTCCATCTGAGACCTGGG-3'

Other clones with same target sequence:

  1. TRCN0000007038