Construct: shRNA TRCN0000315206

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002748.3-3522s21c1
DNA Barcode:
CCAGCGTTTATTGTAGTAAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAPK6 (5597)
Gene Description:
mitogen-activated protein kinase 6
Transcript:
RefSeq NM_002748.3 (NON-CURRENT)
Match location:
Position 3522 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000315206)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCGTTTATTGTAGTAAAC
Hairpin Sequence:
5'-CCGG-CCAGCGTTTATTGTAGTAAAC-CTCGAG-GTTTACTACAATAAACGCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCGTTTATTGTAGTAAACCTCGAGGTTTACTACAATAAACGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCGTTTATTGTAGTAAACCTCGAGGTTTACTACAATAAACGCTGG-3'

Other clones with same target sequence:

  1. TRCN0000195285