Construct: shRNA TRCN0000315225

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001642.2-2428s21c1
DNA Barcode:
CCTGGAGCAGATGCAGATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
APLP2 (334)
Gene Description:
amyloid beta precursor like protein 2
Transcript:
RefSeq NM_001642.2 (NON-CURRENT)
Match location:
Position 2428 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000315225)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGAGCAGATGCAGATTTA
Hairpin Sequence:
5'-CCGG-CCTGGAGCAGATGCAGATTTA-CTCGAG-TAAATCTGCATCTGCTCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGAGCAGATGCAGATTTACTCGAGTAAATCTGCATCTGCTCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGAGCAGATGCAGATTTACTCGAGTAAATCTGCATCTGCTCCAGG-3'

Other clones with same target sequence:

  1. TRCN0000006703