Construct: shRNA TRCN0000315249

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004972.3-4564s21c1
DNA Barcode:
CCCTGACCCTAAATAATACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
JAK2 (3717)
Gene Description:
Janus kinase 2
Transcript:
RefSeq NM_004972.3 (CURRENT)
Match location:
Position 4564 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000315249)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGACCCTAAATAATACAT
Hairpin Sequence:
5'-CCGG-CCCTGACCCTAAATAATACAT-CTCGAG-ATGTATTATTTAGGGTCAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGACCCTAAATAATACATCTCGAGATGTATTATTTAGGGTCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGACCCTAAATAATACATCTCGAGATGTATTATTTAGGGTCAGGG-3'

Other clones with same target sequence:

  1. TRCN0000003178