Construct: shRNA TRCN0000315269

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012300.2-1130s21c1
DNA Barcode:
TATCAGTGGCCTACGAGATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FBXW11 (23291)
Gene Description:
F-box and WD repeat domain containing 11
Transcript:
RefSeq NM_012300.2 (CURRENT)
Match location:
Position 1130 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000315269)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATCAGTGGCCTACGAGATAA
Hairpin Sequence:
5'-CCGG-TATCAGTGGCCTACGAGATAA-CTCGAG-TTATCTCGTAGGCCACTGATA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATCAGTGGCCTACGAGATAACTCGAGTTATCTCGTAGGCCACTGATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATCAGTGGCCTACGAGATAACTCGAGTTATCTCGTAGGCCACTGATA-3'

Other clones with same target sequence:

(none)