Construct: shRNA TRCN0000315359

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133375.3-3200s21c1
DNA Barcode:
CTGGGTAGCTATTTCGCATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DIS3L (115752)
Gene Description:
DIS3 like exosome 3'-5' exoribonuclease
Transcript:
RefSeq NM_133375.3 (NON-CURRENT)
Match location:
Position 3200 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000315359)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGGTAGCTATTTCGCATAT
Hairpin Sequence:
5'-CCGG-CTGGGTAGCTATTTCGCATAT-CTCGAG-ATATGCGAAATAGCTACCCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGGTAGCTATTTCGCATATCTCGAGATATGCGAAATAGCTACCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGGTAGCTATTTCGCATATCTCGAGATATGCGAAATAGCTACCCAG-3'

Other clones with same target sequence:

  1. TRCN0000153834