Construct: shRNA TRCN0000315395

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005921.1-3417s21c1
DNA Barcode:
ATCTCATCATTCCCAATTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP3K1 (4214)
Gene Description:
mitogen-activated protein kinase kinase kinase 1
Transcript:
RefSeq NM_005921.1 (NON-CURRENT)
Match location:
Position 3417 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000315395)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCTCATCATTCCCAATTAAT
Hairpin Sequence:
5'-CCGG-ATCTCATCATTCCCAATTAAT-CTCGAG-ATTAATTGGGAATGATGAGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCTCATCATTCCCAATTAATCTCGAGATTAATTGGGAATGATGAGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCTCATCATTCCCAATTAATCTCGAGATTAATTGGGAATGATGAGAT-3'

Other clones with same target sequence:

(none)