Construct: shRNA TRCN0000315432

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001039582.3-1259s21c1
DNA Barcode:
CCTTCGACAGGGACATCTTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PNCK (139728)
Gene Description:
pregnancy up-regulated nonubiquitous CaM kinase
Transcript:
RefSeq NM_001039582.3 (CURRENT)
Match location:
Position 1259 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000315432)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCGACAGGGACATCTTAG
Hairpin Sequence:
5'-CCGG-CCTTCGACAGGGACATCTTAG-CTCGAG-CTAAGATGTCCCTGTCGAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCGACAGGGACATCTTAGCTCGAGCTAAGATGTCCCTGTCGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCGACAGGGACATCTTAGCTCGAGCTAAGATGTCCCTGTCGAAGG-3'

Other clones with same target sequence:

(none)