Construct: shRNA TRCN0000315465

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009818.1-912s21c1
DNA Barcode:
CGCTCTCAACAACTTTGATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ctnna1 (12385)
Gene Description:
catenin (cadherin associated protein), alpha 1
Transcript:
RefSeq NM_009818.1 (CURRENT)
Match location:
Position 912 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000315465)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTCTCAACAACTTTGATAA
Hairpin Sequence:
5'-CCGG-CGCTCTCAACAACTTTGATAA-CTCGAG-TTATCAAAGTTGTTGAGAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTCTCAACAACTTTGATAACTCGAGTTATCAAAGTTGTTGAGAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTCTCAACAACTTTGATAACTCGAGTTATCAAAGTTGTTGAGAGCG-3'

Other clones with same target sequence:

  1. TRCN0000108743