Construct: shRNA TRCN0000315528

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010629.2-1913s21c1
DNA Barcode:
CGGTGTCTATGGATGACTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kifap3 (16579)
Gene Description:
kinesin-associated protein 3
Transcript:
RefSeq NM_010629.2 (NON-CURRENT)
Match location:
Position 1913 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000315528)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTGTCTATGGATGACTCTT
Hairpin Sequence:
5'-CCGG-CGGTGTCTATGGATGACTCTT-CTCGAG-AAGAGTCATCCATAGACACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTGTCTATGGATGACTCTTCTCGAGAAGAGTCATCCATAGACACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTGTCTATGGATGACTCTTCTCGAGAAGAGTCATCCATAGACACCG-3'

Other clones with same target sequence:

  1. TRCN0000090946