Construct: shRNA TRCN0000315546

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019952.3-467s21c1
DNA Barcode:
CCACAGCTGAACTCCGACGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Clcf1 (56708)
Gene Description:
cardiotrophin-like cytokine factor 1
Transcript:
RefSeq NM_019952.3 (NON-CURRENT)
Match location:
Position 467 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000315546)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAGCTGAACTCCGACGTA
Hairpin Sequence:
5'-CCGG-CCACAGCTGAACTCCGACGTA-CTCGAG-TACGTCGGAGTTCAGCTGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAGCTGAACTCCGACGTACTCGAGTACGTCGGAGTTCAGCTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAGCTGAACTCCGACGTACTCGAGTACGTCGGAGTTCAGCTGTGG-3'

Other clones with same target sequence:

  1. TRCN0000088931