Construct: shRNA TRCN0000315588

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133350.1-817s21c1
DNA Barcode:
CGAGATTTCTATTTCAGCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mapre3 (100732)
Gene Description:
microtubule-associated protein, RP/EB family, member 3
Transcript:
RefSeq NM_133350.1 (NON-CURRENT)
Match location:
Position 817 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000315588)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGATTTCTATTTCAGCAAA
Hairpin Sequence:
5'-CCGG-CGAGATTTCTATTTCAGCAAA-CTCGAG-TTTGCTGAAATAGAAATCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGATTTCTATTTCAGCAAACTCGAGTTTGCTGAAATAGAAATCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGATTTCTATTTCAGCAAACTCGAGTTTGCTGAAATAGAAATCTCG-3'

Other clones with same target sequence:

  1. TRCN0000087786