Construct: shRNA TRCN0000315697

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001316.2-1150s21c1
DNA Barcode:
CGCTGACAAGTATCTGTGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSE1L (1434)
Gene Description:
chromosome segregation 1 like
Transcript:
RefSeq NM_001316.2 (NON-CURRENT)
Match location:
Position 1150 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000315697)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTGACAAGTATCTGTGAAA
Hairpin Sequence:
5'-CCGG-CGCTGACAAGTATCTGTGAAA-CTCGAG-TTTCACAGATACTTGTCAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTGACAAGTATCTGTGAAACTCGAGTTTCACAGATACTTGTCAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTGACAAGTATCTGTGAAACTCGAGTTTCACAGATACTTGTCAGCG-3'

Other clones with same target sequence:

  1. TRCN0000061790