Construct: shRNA TRCN0000315710

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020123.3-1624s21c1
DNA Barcode:
CACGTCTTTCTGGGCATATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TM9SF3 (56889)
Gene Description:
transmembrane 9 superfamily member 3
Transcript:
RefSeq NM_020123.3 (NON-CURRENT)
Match location:
Position 1624 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000315710)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACGTCTTTCTGGGCATATAA
Hairpin Sequence:
5'-CCGG-CACGTCTTTCTGGGCATATAA-CTCGAG-TTATATGCCCAGAAAGACGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACGTCTTTCTGGGCATATAACTCGAGTTATATGCCCAGAAAGACGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACGTCTTTCTGGGCATATAACTCGAGTTATATGCCCAGAAAGACGTG-3'

Other clones with same target sequence:

  1. TRCN0000059372