Construct: shRNA TRCN0000315723

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005556.3-778s21c1
DNA Barcode:
CCTGAATGATGAGATCAACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KRT7 (3855)
Gene Description:
keratin 7
Transcript:
RefSeq NM_005556.3 (NON-CURRENT)
Match location:
Position 778 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000315723)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAATGATGAGATCAACTT
Hairpin Sequence:
5'-CCGG-CCTGAATGATGAGATCAACTT-CTCGAG-AAGTTGATCTCATCATTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAATGATGAGATCAACTTCTCGAGAAGTTGATCTCATCATTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAATGATGAGATCAACTTCTCGAGAAGTTGATCTCATCATTCAGG-3'

Other clones with same target sequence:

  1. TRCN0000116348