Construct: shRNA TRCN0000315759

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018686.3-803s21c1
DNA Barcode:
GCTGAACATAGTGTGGATATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CMAS (55907)
Gene Description:
cytidine monophosphate N-acetylneuraminic acid synthetase
Transcript:
RefSeq NM_018686.3 (NON-CURRENT)
Match location:
Position 803 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000315759)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAACATAGTGTGGATATA
Hairpin Sequence:
5'-CCGG-GCTGAACATAGTGTGGATATA-CTCGAG-TATATCCACACTATGTTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAACATAGTGTGGATATACTCGAGTATATCCACACTATGTTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAACATAGTGTGGATATACTCGAGTATATCCACACTATGTTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000045460