Construct: shRNA TRCN0000315778

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000821.4-511s21c1
DNA Barcode:
CCGGATAAGCTGTGTGTTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GGCX (2677)
Gene Description:
gamma-glutamyl carboxylase
Transcript:
RefSeq NM_000821.4 (NON-CURRENT)
Match location:
Position 511 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000315778)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGATAAGCTGTGTGTTATT
Hairpin Sequence:
5'-CCGG-CCGGATAAGCTGTGTGTTATT-CTCGAG-AATAACACACAGCTTATCCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGATAAGCTGTGTGTTATTCTCGAGAATAACACACAGCTTATCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGATAAGCTGTGTGTTATTCTCGAGAATAACACACAGCTTATCCGG-3'

Other clones with same target sequence:

  1. TRCN0000078520