Construct: shRNA TRCN0000315822

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006196.3-1403s21c1
DNA Barcode:
CCCATGATCCAACTGTGTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCBP1 (5093)
Gene Description:
poly(rC) binding protein 1
Transcript:
RefSeq NM_006196.3 (NON-CURRENT)
Match location:
Position 1403 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000315822)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATGATCCAACTGTGTAAT
Hairpin Sequence:
5'-CCGG-CCCATGATCCAACTGTGTAAT-CTCGAG-ATTACACAGTTGGATCATGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATGATCCAACTGTGTAATCTCGAGATTACACAGTTGGATCATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATGATCCAACTGTGTAATCTCGAGATTACACAGTTGGATCATGGG-3'

Other clones with same target sequence:

  1. TRCN0000074668