Construct: shRNA TRCN0000315835

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002417.4-12011s21c1
DNA Barcode:
CCCATTAAATACAAGCTGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MKI67 (4288)
Gene Description:
marker of proliferation Ki-67
Transcript:
RefSeq NM_002417.4 (NON-CURRENT)
Match location:
Position 12011 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000315835)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATTAAATACAAGCTGTTT
Hairpin Sequence:
5'-CCGG-CCCATTAAATACAAGCTGTTT-CTCGAG-AAACAGCTTGTATTTAATGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATTAAATACAAGCTGTTTCTCGAGAAACAGCTTGTATTTAATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATTAAATACAAGCTGTTTCTCGAGAAACAGCTTGTATTTAATGGG-3'

Other clones with same target sequence:

  1. TRCN0000117957