Construct: shRNA TRCN0000315881

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181581.1-502s21c1
DNA Barcode:
CCAATGATTGTTGCCGCTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DUS4L (11062)
Gene Description:
dihydrouridine synthase 4 like
Transcript:
RefSeq NM_181581.1 (NON-CURRENT)
Match location:
Position 502 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000315881)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAATGATTGTTGCCGCTGAT
Hairpin Sequence:
5'-CCGG-CCAATGATTGTTGCCGCTGAT-CTCGAG-ATCAGCGGCAACAATCATTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAATGATTGTTGCCGCTGATCTCGAGATCAGCGGCAACAATCATTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAATGATTGTTGCCGCTGATCTCGAGATCAGCGGCAACAATCATTGG-3'

Other clones with same target sequence:

  1. TRCN0000064854