Construct: shRNA TRCN0000316008

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026496.3-1664s21c1
DNA Barcode:
CGTCCTTGTTAAGCGGATGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Grhl2 (252973)
Gene Description:
grainyhead-like 2 (Drosophila)
Transcript:
RefSeq NM_026496.3 (NON-CURRENT)
Match location:
Position 1664 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000316008)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCCTTGTTAAGCGGATGTT
Hairpin Sequence:
5'-CCGG-CGTCCTTGTTAAGCGGATGTT-CTCGAG-AACATCCGCTTAACAAGGACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCCTTGTTAAGCGGATGTTCTCGAGAACATCCGCTTAACAAGGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCCTTGTTAAGCGGATGTTCTCGAGAACATCCGCTTAACAAGGACG-3'

Other clones with same target sequence:

  1. TRCN0000084224