Construct: shRNA TRCN0000316157

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021449.2-747s21c1
DNA Barcode:
CGCTGGCTGTATTCATTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Crbn (58799)
Gene Description:
cereblon
Transcript:
RefSeq NM_021449.2 (NON-CURRENT)
Match location:
Position 747 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000316157)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTGGCTGTATTCATTATAT
Hairpin Sequence:
5'-CCGG-CGCTGGCTGTATTCATTATAT-CTCGAG-ATATAATGAATACAGCCAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTGGCTGTATTCATTATATCTCGAGATATAATGAATACAGCCAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTGGCTGTATTCATTATATCTCGAGATATAATGAATACAGCCAGCG-3'

Other clones with same target sequence:

  1. TRCN0000113344