Construct: shRNA TRCN0000316231

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133255.1-449s21c1
DNA Barcode:
CTGTGCTATCAGTTGTGAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hook2 (170833)
Gene Description:
hook microtubule tethering protein 2
Transcript:
RefSeq NM_133255.1 (NON-CURRENT)
Match location:
Position 449 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000316231)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTGCTATCAGTTGTGAGAA
Hairpin Sequence:
5'-CCGG-CTGTGCTATCAGTTGTGAGAA-CTCGAG-TTCTCACAACTGATAGCACAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTGCTATCAGTTGTGAGAACTCGAGTTCTCACAACTGATAGCACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTGCTATCAGTTGTGAGAACTCGAGTTCTCACAACTGATAGCACAG-3'

Other clones with same target sequence:

  1. TRCN0000111733