Construct: shRNA TRCN0000316298

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007943.2-2877s21c1
DNA Barcode:
CCTATGAGCATGGGATACAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eps15 (13858)
Gene Description:
epidermal growth factor receptor pathway substrate 15
Transcript:
RefSeq NM_007943.2 (NON-CURRENT)
Match location:
Position 2877 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000316298)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTATGAGCATGGGATACAAA
Hairpin Sequence:
5'-CCGG-CCTATGAGCATGGGATACAAA-CTCGAG-TTTGTATCCCATGCTCATAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTATGAGCATGGGATACAAACTCGAGTTTGTATCCCATGCTCATAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTATGAGCATGGGATACAAACTCGAGTTTGTATCCCATGCTCATAGG-3'

Other clones with same target sequence:

  1. TRCN0000111720