Construct: shRNA TRCN0000316327

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033320.4-2604s21c1
DNA Barcode:
GCTGCATTTCATTTCATACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Glce (93683)
Gene Description:
glucuronyl C5-epimerase
Transcript:
RefSeq NM_033320.4 (CURRENT)
Match location:
Position 2604 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000316327)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCATTTCATTTCATACTA
Hairpin Sequence:
5'-CCGG-GCTGCATTTCATTTCATACTA-CTCGAG-TAGTATGAAATGAAATGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCATTTCATTTCATACTACTCGAGTAGTATGAAATGAAATGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCATTTCATTTCATACTACTCGAGTAGTATGAAATGAAATGCAGC-3'

Other clones with same target sequence:

  1. TRCN0000099235