Construct: shRNA TRCN0000316447

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012039.1-1225s21c1
DNA Barcode:
GCTCGCAATATCAATTCTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zw10 (26951)
Gene Description:
zw10 kinetochore protein
Transcript:
RefSeq NM_012039.1 (NON-CURRENT)
Match location:
Position 1225 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000316447)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCGCAATATCAATTCTCAT
Hairpin Sequence:
5'-CCGG-GCTCGCAATATCAATTCTCAT-CTCGAG-ATGAGAATTGATATTGCGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCGCAATATCAATTCTCATCTCGAGATGAGAATTGATATTGCGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCGCAATATCAATTCTCATCTCGAGATGAGAATTGATATTGCGAGC-3'

Other clones with same target sequence:

  1. TRCN0000098187