Construct: shRNA TRCN0000316586

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016697.2-808s21c1
DNA Barcode:
GCCCTGAATCTCGGAATTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpc3 (14734)
Gene Description:
glypican 3
Transcript:
RefSeq NM_016697.2 (NON-CURRENT)
Match location:
Position 808 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000316586)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTGAATCTCGGAATTGAA
Hairpin Sequence:
5'-CCGG-GCCCTGAATCTCGGAATTGAA-CTCGAG-TTCAATTCCGAGATTCAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTGAATCTCGGAATTGAACTCGAGTTCAATTCCGAGATTCAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTGAATCTCGGAATTGAACTCGAGTTCAATTCCGAGATTCAGGGC-3'

Other clones with same target sequence:

  1. TRCN0000109456