Construct: shRNA TRCN0000316592

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031159.3-620s21c1
DNA Barcode:
CCACGAGTTTGAAGTCTTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Apobec1 (11810)
Gene Description:
apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1
Transcript:
RefSeq NM_031159.3 (CURRENT)
Match location:
Position 620 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000316592)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACGAGTTTGAAGTCTTCTT
Hairpin Sequence:
5'-CCGG-CCACGAGTTTGAAGTCTTCTT-CTCGAG-AAGAAGACTTCAAACTCGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACGAGTTTGAAGTCTTCTTCTCGAGAAGAAGACTTCAAACTCGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACGAGTTTGAAGTCTTCTTCTCGAGAAGAAGACTTCAAACTCGTGG-3'

Other clones with same target sequence:

  1. TRCN0000098682