Construct: shRNA TRCN0000316739

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018788.2-2147s21c1
DNA Barcode:
GCCATGATTAGGACTCGAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Extl3 (54616)
Gene Description:
exostoses (multiple)-like 3
Transcript:
RefSeq NM_018788.2 (NON-CURRENT)
Match location:
Position 2147 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000316739)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATGATTAGGACTCGAATT
Hairpin Sequence:
5'-CCGG-GCCATGATTAGGACTCGAATT-CTCGAG-AATTCGAGTCCTAATCATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATGATTAGGACTCGAATTCTCGAGAATTCGAGTCCTAATCATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATGATTAGGACTCGAATTCTCGAGAATTCGAGTCCTAATCATGGC-3'

Other clones with same target sequence:

  1. TRCN0000093747