Construct: shRNA TRCN0000316774

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016759.2-1610s21c1
DNA Barcode:
CCTGGTTCCTTCTGATTTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rundc3a (51799)
Gene Description:
RUN domain containing 3A
Transcript:
RefSeq NM_016759.2 (NON-CURRENT)
Match location:
Position 1610 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000316774)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGTTCCTTCTGATTTCTT
Hairpin Sequence:
5'-CCGG-CCTGGTTCCTTCTGATTTCTT-CTCGAG-AAGAAATCAGAAGGAACCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGTTCCTTCTGATTTCTTCTCGAGAAGAAATCAGAAGGAACCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGTTCCTTCTGATTTCTTCTCGAGAAGAAATCAGAAGGAACCAGG-3'

Other clones with same target sequence:

  1. TRCN0000106090