Construct: shRNA TRCN0000316803

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028024.2-783s21c1
DNA Barcode:
GCCACCTTTGTCCTCTCTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nkiras2 (71966)
Gene Description:
NFKB inhibitor interacting Ras-like protein 2
Transcript:
RefSeq NM_028024.2 (CURRENT)
Match location:
Position 783 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000316803)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACCTTTGTCCTCTCTTAA
Hairpin Sequence:
5'-CCGG-GCCACCTTTGTCCTCTCTTAA-CTCGAG-TTAAGAGAGGACAAAGGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACCTTTGTCCTCTCTTAACTCGAGTTAAGAGAGGACAAAGGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACCTTTGTCCTCTCTTAACTCGAGTTAAGAGAGGACAAAGGTGGC-3'

Other clones with same target sequence:

  1. TRCN0000077523