Construct: shRNA TRCN0000316814

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028717.4-2526s21c1
DNA Barcode:
CCTGCCATTGATTTCCTAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Als2 (74018)
Gene Description:
amyotrophic lateral sclerosis 2 (juvenile)
Transcript:
RefSeq NM_028717.4 (NON-CURRENT)
Match location:
Position 2526 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000316814)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCCATTGATTTCCTAAAT
Hairpin Sequence:
5'-CCGG-CCTGCCATTGATTTCCTAAAT-CTCGAG-ATTTAGGAAATCAATGGCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCCATTGATTTCCTAAATCTCGAGATTTAGGAAATCAATGGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCCATTGATTTCCTAAATCTCGAGATTTAGGAAATCAATGGCAGG-3'

Other clones with same target sequence:

  1. TRCN0000041029