Construct: shRNA TRCN0000316821

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023742.1-1919s21c1
DNA Barcode:
CGGCAATGCTACCTGCCAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dtx2 (74198)
Gene Description:
deltex 2, E3 ubiquitin ligase
Transcript:
RefSeq NM_023742.1 (NON-CURRENT)
Match location:
Position 1919 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000316821)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCAATGCTACCTGCCAGAT
Hairpin Sequence:
5'-CCGG-CGGCAATGCTACCTGCCAGAT-CTCGAG-ATCTGGCAGGTAGCATTGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCAATGCTACCTGCCAGATCTCGAGATCTGGCAGGTAGCATTGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCAATGCTACCTGCCAGATCTCGAGATCTGGCAGGTAGCATTGCCG-3'

Other clones with same target sequence:

  1. TRCN0000041092