Construct: shRNA TRCN0000316943

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145853.2-2873s21c1
DNA Barcode:
GCTCCAGAAATAACCAGCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tpcn1 (252972)
Gene Description:
two pore channel 1
Transcript:
RefSeq NM_145853.2 (CURRENT)
Match location:
Position 2873 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000316943)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCCAGAAATAACCAGCTTA
Hairpin Sequence:
5'-CCGG-GCTCCAGAAATAACCAGCTTA-CTCGAG-TAAGCTGGTTATTTCTGGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCCAGAAATAACCAGCTTACTCGAGTAAGCTGGTTATTTCTGGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCCAGAAATAACCAGCTTACTCGAGTAAGCTGGTTATTTCTGGAGC-3'

Other clones with same target sequence:

  1. TRCN0000069993