Construct: shRNA TRCN0000316974

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133347.1-3467s21c1
DNA Barcode:
GACATATTTCATGGCCGTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dhx30 (72831)
Gene Description:
DEAH (Asp-Glu-Ala-His) box polypeptide 30
Transcript:
RefSeq NM_133347.1 (NON-CURRENT)
Match location:
Position 3467 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000316974)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACATATTTCATGGCCGTCAA
Hairpin Sequence:
5'-CCGG-GACATATTTCATGGCCGTCAA-CTCGAG-TTGACGGCCATGAAATATGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACATATTTCATGGCCGTCAACTCGAGTTGACGGCCATGAAATATGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACATATTTCATGGCCGTCAACTCGAGTTGACGGCCATGAAATATGTC-3'

Other clones with same target sequence:

  1. TRCN0000113048